Tesi di Dottorato
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Item Dynamic Methods for Monitoring Structural Health: analytical and experimental aspects(Università della Calabria, 2018-11) Miceli, Angela; Zinno, Raffaele; Carbone, VincenzoItem GPER mediates the up-regulation of acid synthase (fasn) intuced by 17B-estradiol in cancer cell and in cancer-associated fibroblasts (CAFs)(2012-11-26) Santolla, Maria Francesca; Maggiolini, Marcello; Sisci, DiegoActivation of lipid metabolism is an early event in carcinogenesis and a central hallmark of many tumors. Fatty acid synthase (FASN) is a key lipogenic enzyme catalyzing the terminal steps in the de novo biogenesis of fatty acids. In cancer cells, FASN may act as a metabolic oncogene given that it confers growth and survival advantages to these cells, whereas its inhibition effectively and selectively kills tumor cells. Hormones like estrogens and growth factors contribute to the transcriptional regulation of FASN expression also through the activation of downstream signaling and a crosstalk among diverse transduction pathways. In this study, we demonstrate for the first time that 17β-estradiol (E2) and the selective GPER ligand G-1 regulate FASN expression and activity through the GPER-mediated signaling which involved the EGFR/ERK/c-fos/AP1 transduction pathway, as ascertained by using specific pharmacological inhibitors, performing gene-silencing experiments and ChiP assays in breast SkBr3, colorectal LoVo, hepatocarcinoma HepG2 cancer cells and breast cancerassociated fibroblasts (CAFs). In addition, the proliferative effects induced by E2 and G-1 in these cells involved FASN as the inhibitor of its activity, named cerulenin, abolished the growth response to both ligands. Our data suggest that GPER may be included among the transduction mediators involved by estrogens in regulating FASN expression and activity in cancer cells and CAFs that strongly contribute to cancer progression.Item <> costruzione di possibili profili di sostenibilità applicati a scala di quartiere.(2013-11-29) Manfredi, Emilia; Pantano, Pietro; Rossi, FrancescoItem Simulation-based optimization in port logistics(2017) Mazza, Rina Mary; Grandinetti, Lucio; Legato, PasqualeItem Preparation of modified α-amino acids, useful building blocks for peptide sinthesis(2010-12-15) De Marco, Rosalia; Liguori, Angelo; Gabriele, BartoloItem Metodi e tool per l'ottimizzazione della calibrazione e della validazione dei sistemi di controllo motore(2012-09-29) Guzzo, Alessandra; Rizzuti, Sergio; Bova, Sergio; Riegel, AlessandroItem Towards more sustainable organic processes : heterocyclizations in non-conventional solvents(2017-09-19) Maner, Asif S.; Carbone, Vincenzo; Gabriele, Bartolo; Mancuso, RaffaellaThis thesis reports the synthesis of important heterocyclic derivatives by iodocyclization, carbonylation and cycloisomerization reactions in Non-Conventionl solvents like deep eutectic solvents (DES) and Ionic Liquids (ILs). In chapter one general aspects of green and sustainable chemistry and introduction to eco-friendly green solvents such as water, DES, ScCO2 and ILs are described. Carbonylation processes, their advantages, types were described along with the application of transition metal catalysis in the carbonylation reactions with mechanistic approaches discussed. In chapter two, we describe a convenient and general method for the synthesis of substituted thiophenes through heterocyclodehydration and iodocyclization of readily available 1-mercapto-3-alkyn-2-ols in DES as the solvents. In chapter three we discuss a convenient carbonylative approach to 2-oxazolidinone derivatives carried out in an ionic liquid as the solvent (EmimEtSO4) is presented. It is based on the sequential concatenation of two catalytic cycles, both catalyzed by the same metal species (auto-tandem catalysis). In chapter four we present iodocyclization reactions to obtain iodinated isobenzofuranones and isochromenones by iodolactonization of 2-alkynyl benzoic acids in ionic liquids. In particular here we have developed divergent syntheses of (E)-3-(iodoalkylidene) isobenzofuran-1(3H)-ones and 4-iodo-1H-isochromen-1-ones by base-free Iodolactonization of 2-alkynylbenzoic acids in ionic liquids. In chapter five we report the cycloisomerization of readily available 2-alkynylbenzoic acids using an ionic liquid as the reaction medium in the presence of CuCl2 as a simple and inexpensive catalyst. Although in principle two different cyclization pathways can be followed, leading to either 5-exo-dig mode or 6-endo-dig mode, we have found that substrates bearing an aryl group on the triple bond or a terminal triple bond can be selectively converted into the isobenzofuranone derivatives, using N-ethyl-N-methylmorpholinium dicyanamide (Mor1,2N(CN)2) as the solvent. On the other hand, and in a complementary manner, substrates substituted with an alkyl or an alkenyl group on the triple bond selectively led to isochromenones when the reaction was carried out EmimEtSO4 and with excellent recyclability of the catalyst/ionic liquid system.Item Improving oilfield performance enabling horizontal drilling techniques: developments and optimization of standard and special measures for a real case study(2017-11-13) Al Taie, Amer Abdulhakim; Carbone, Vincenzo; Romagnoli, RaffaeleDrilling technique is a crucial issue to pay attention to. Drilling a horizontal well has a great interest to the oil and gas industry since nowadays it provides attractive means for improving both production rate and yet the recovery efficiency. The great improvements in drilling technology make it possible to drill horizontally no matter how complex are the trajectories and how deep it is suppose to reach. This study aims at presenting the optimal design aspects of a horizontal well. Design aspects include the selection of bit and casing sizes, detection of setting depths and drilling fluid density, casing, hydraulics, well profile, and construction of drill string simulator. When many vertical wells exist, an oil field named (Z14 field) should be designated to have a short radius horizontal well able to increasing the productivity and to promote the developing of the field itself. A single build profile with build rate 90 deg/100ft is constructed based on geological data. A drill string simulator composed of soft-string model and buckling tendency is constructed to predict torque and drag of string for six operating conditions. These conditions are pick-up, slack-off, sliding, pick-up with rotation, slack-off with rotation and drilling with rotation. Results of loads analysis showed that the suggested drill string can be used without exceeding torsional, tensile and buckling strengths. Analysis of single build profile showed that the torque and drag while drilling horizontal well could be minimized by drilling with low build rate, employing lighter pipe, and improving the lubricating capability with oil base mud (low friction forces). A finite element model was constructed to predict inclination tendency for multistabilizer rotary BHA in three dimensions, static condition. The bottom hole assembly was idealized with beam element capable of resisting axial forces, bending moments about the two principal axes, and twisting moments about its centroidal axis. Bit and stabilizer were treated as contact point and restricted from movement in all directions. Each element is loaded with gravity and normal contact forces. Model validation showed closer agreement between the model and Jiazhi's method (analytic) for slick, single, and two stabilizers BHA, compared to Akgun results. Predictions with finite element model showed that for building assembly, the weight on bit had small effect on bit side force especially in high angle wells. Also inclination tendency (building, dropping) would depend on position of the stabilizer, diameter of drillcollar behind the bit, and number of stabilizers.Item Rischio sismico ambientale e degli edifici: analisi tecnica HVSR - Aspetti teorici e aspetti sperimentali(2017-07-17) Magarò, Floriana; Carbone, Vincenzo; Zinno, RaffaeleItem TiO2 nanotubes in nanotechnologies(2017-10-10) Jimenez, Leticia; Versace, Carlo